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m3003 experimental models  (New England Biolabs)


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    New England Biolabs m3003 experimental models
    M3003 Experimental Models, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 4308 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/m3003+experimental+models/Luna+Universal+qPCR+Master+Mix/pm40683250-298-15-10
    Average 99 stars, based on 4308 article reviews
    m3003 experimental models - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Real-time Polymerase Chain Reaction:

    Article Title: Reprogramming of endolysosomes for melanogenesis in BLOC-1-deficient melanocytes.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies HA tag Abcam Cat. # ab9110; RRID:AB_307019 TYRP1 ATCC Cat. # TA99/Mel-5 LAMP2 Developmental Studies Hybridoma Bank Cat. # GL2A7; RRID:AB_2314734 Goat anti-mouse AlexaFluor488 Invitrogen Cat. # A-11001; RRID:AB_2534069 Goat anti-rabbit AlexaFluor568 Invitrogen Cat. # A-11011; RRID:AB_143157 Goat anti-rat AlexaFluor568 Invitrogen Cat. # A-11077; RRID:AB_141874 αPep7h (anti-TYR) Marks lab N/A αPep13h (anti-PMEL) Marks lab N/A TYRP1 Santa Cruz Biotechnology Cat. # sc-166857; RRID:AB_2211147 Actin Sigma-Aldrich Cat. # A5060; RRID:AB_476738 γ-tubulin Sigma Cat. # T6557; RRID:AB_477584 Goat anti-mouse IgG-IRDye 800CW Li-Cor Cat. # 926-32210; RRID:AB_621842 Goat anti-Rabbit IgG-IR Dye 680R Li-Cor Cat. # 926-68071; RRID:AB_10956166 Donkey anti-mouse IgG (H+L) AlexaFluor790 Jackson ImmunoResearch Cat. # 715-655-151; RRID:AB_2340871 Donkey anti-rabbit IgG (H+L) AlexaFluor680 Jackson ImmunoResearch Cat. # 711-625-152; RRID:AB_2340627 Chemicals, peptides, and recombinant proteins 12-0-tetradecanoyl phorbol acetate (TPA) Sigma-Aldrich Cat. # P8139 4-Norleucine,7-D-phenylalanine-alphamelanocyte-stimulating hormone (NDP-MSH) Sigma-Aldrich Cat. # M8764 Forskolin Sigma-Aldrich Cat. # F6886 Cholera toxin Sigma-Aldrich Cat. # C8052 Bafilomycin A1 Sigma-Aldrich Cat. # B1793 Cycloheximide Sigma-Aldrich Cat. # C1988-1G L-Methionine methyl ester hydrochloride Thermo Scientific Chemicals Cat. # A15952.14 Monensin Sigma-Aldrich Cat. # M5273 Nigericin Sigma-Aldrich Cat. # N7143 Sodium cacodylate Sigma-Alrich Cat. # C0250 Osmium Tetroxide Agar Scientific Cat. # AGR1019 Propylene oxide Sigma-Aldrich Cat. # 82320 Agar 100 Resin Kit Agar Scientific Cat. # AGR1031 Uranyl acetate Agar Scientific Cat. # AGR1260A Lead citrate Agar Scientific Cat. # AGR1210 Dodecenyl succinic anhydride Agar Scientific Cat. #R1053 Methyl nadic anhydride Agar Scientific Cat. #R1083 Benzyl dimethylamine Agar Scientific Cat. #AGR1062 Potassium ferricyanide Sigma-Aldrich Cat. # 702587 Tannic acid TAAB Laboratories Equipment Limited Cat. # T046 Sodium sulphate Sigma-Aldrich Cat. # 239313 TAAB 812 Resin kit TAAB Laboratories Equipment Limited Cat. # T024/1 Lipofectamine 3000 ThermoFisher Scientific Cat. #L3000015 Critical commercial assays RNeasy Plus Mini Kit Qiagen Cat. # 74134 LunaScript RT SuperMix Kit New England Biolabs Cat. # M3010 (Continued on next page) Current Biology 35, 3570–3586.e1–e7, August 4, 2025 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Luna Universal qPCR Master Mix New England Biolabs Cat. # M3003 Experimental models: Cell lines melan-pa: Bloc1S6 pa/pa Functional Genomics Cell Bank at City St. George’s University of London N/A melan-mu: Bloc1S5 mu/mu Functional Genomics Cell Bank at City St. George’s University of London N/A melan-a: a/a Functional Genomics Cell Bank at City St. George’s University of London N/A melan-Ink4a-Arf1: Ink4a-Arf -/- Functional Genomics Cell Bank at City St. George’s University of London N/A melan-c2: Tyr c-2J /Tyr c-2J Functional Genomics Cell Bank at City St. George’s University of London N/A melan-mu:MuHA Functional Genomics Cell Bank at City St. George’s University of London N/A Oligonucleotides TYR Fwd: 5 ’ CGA TGG AAC ACC TGA GGG AC Integrated DNA Technologies N/A TYR Rev:5 ’ AGT GGA CTG GCA AAT CCT TCC Integrated DNA Technologies N/A Atp5b primer set (Anchor nucelotide: 115) Primer Design Ltd. geNorm kit (Discontinued) Ubc primer set (Anchor nucelotide: 2627) Primer Design Ltd. geNorm kit (Discontinued) B2m primer set (Anchor nucelotide: 202) Primer Design Ltd. geNorm kit (Discontinued) Sdha primer set (Anchor nucelotide: 2018) Primer Design Ltd. geNorm kit (Discontinued) Recombinant DNA pCR3/OCA2-HA Michael Marks N/A pCI-HA-SLC45A2 Michael Marks N/A pN1-LAMP1-tdTomato Tom Carter N/A Software and algorithms GraphPad Prism (version 10.2.1) GraphPad https://www.graphpad.com/ ImageJ NIH https://imagej.nih.gov/ij/ Photoshop CS6 (Version 13.0) Adobe Systems N/A NIS Elements (Version 4.2) Nikon N/A Image Studio (Version 5.2) Li-Cor N/A AMT Capture Engine software (Version V602.580.20d) Advanced Microscopy Techniques N/A Other RPMI-1640 Medium Sigma-Alrich Cat. # R7509 Fetal Bovine Serum Gibco Cat. # 10270106 Dextran, Oregon Green 488; 10,000 MW Invitrogen Cat. # D7170 L-Glutamine Gibco Cat. # 25030081 Penicillin-Streptomycin (10,000 U/mL) Gibco Cat. # 15140122 HEPES Gibco Cat. # 15630056 Protease Inhibitor cocktail Roche Cat. # 11697498001 Immobilon-FL PVDF Membrane Millipore Cat. # IPFL85R Nitrocellulose membrane, 0.45 μm BioRad Cat. # 1620115 CitiFluor AF1 mounting solution Agar Scientific Cat. # AGR1320 Copper mesh grids Agar Scientific Cat. # AGG2415C 35 mm Dish with No. 1.5 Gridded Coverslip, 14 mm Glass Diameter MatTek Cat. # P35G-1.5-14-C-GRD CitiFluor AF1 mounting solution Agar Scientific Cat. #AGR1320 Black 96 well plate Greiner Bio-One Cat. #655900 e2 Current Biology 35, 3570–3586.e1–e7, August 4, 2025 ..

    Functional Assay:

    Article Title: Reprogramming of endolysosomes for melanogenesis in BLOC-1-deficient melanocytes.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies HA tag Abcam Cat. # ab9110; RRID:AB_307019 TYRP1 ATCC Cat. # TA99/Mel-5 LAMP2 Developmental Studies Hybridoma Bank Cat. # GL2A7; RRID:AB_2314734 Goat anti-mouse AlexaFluor488 Invitrogen Cat. # A-11001; RRID:AB_2534069 Goat anti-rabbit AlexaFluor568 Invitrogen Cat. # A-11011; RRID:AB_143157 Goat anti-rat AlexaFluor568 Invitrogen Cat. # A-11077; RRID:AB_141874 αPep7h (anti-TYR) Marks lab N/A αPep13h (anti-PMEL) Marks lab N/A TYRP1 Santa Cruz Biotechnology Cat. # sc-166857; RRID:AB_2211147 Actin Sigma-Aldrich Cat. # A5060; RRID:AB_476738 γ-tubulin Sigma Cat. # T6557; RRID:AB_477584 Goat anti-mouse IgG-IRDye 800CW Li-Cor Cat. # 926-32210; RRID:AB_621842 Goat anti-Rabbit IgG-IR Dye 680R Li-Cor Cat. # 926-68071; RRID:AB_10956166 Donkey anti-mouse IgG (H+L) AlexaFluor790 Jackson ImmunoResearch Cat. # 715-655-151; RRID:AB_2340871 Donkey anti-rabbit IgG (H+L) AlexaFluor680 Jackson ImmunoResearch Cat. # 711-625-152; RRID:AB_2340627 Chemicals, peptides, and recombinant proteins 12-0-tetradecanoyl phorbol acetate (TPA) Sigma-Aldrich Cat. # P8139 4-Norleucine,7-D-phenylalanine-alphamelanocyte-stimulating hormone (NDP-MSH) Sigma-Aldrich Cat. # M8764 Forskolin Sigma-Aldrich Cat. # F6886 Cholera toxin Sigma-Aldrich Cat. # C8052 Bafilomycin A1 Sigma-Aldrich Cat. # B1793 Cycloheximide Sigma-Aldrich Cat. # C1988-1G L-Methionine methyl ester hydrochloride Thermo Scientific Chemicals Cat. # A15952.14 Monensin Sigma-Aldrich Cat. # M5273 Nigericin Sigma-Aldrich Cat. # N7143 Sodium cacodylate Sigma-Alrich Cat. # C0250 Osmium Tetroxide Agar Scientific Cat. # AGR1019 Propylene oxide Sigma-Aldrich Cat. # 82320 Agar 100 Resin Kit Agar Scientific Cat. # AGR1031 Uranyl acetate Agar Scientific Cat. # AGR1260A Lead citrate Agar Scientific Cat. # AGR1210 Dodecenyl succinic anhydride Agar Scientific Cat. #R1053 Methyl nadic anhydride Agar Scientific Cat. #R1083 Benzyl dimethylamine Agar Scientific Cat. #AGR1062 Potassium ferricyanide Sigma-Aldrich Cat. # 702587 Tannic acid TAAB Laboratories Equipment Limited Cat. # T046 Sodium sulphate Sigma-Aldrich Cat. # 239313 TAAB 812 Resin kit TAAB Laboratories Equipment Limited Cat. # T024/1 Lipofectamine 3000 ThermoFisher Scientific Cat. #L3000015 Critical commercial assays RNeasy Plus Mini Kit Qiagen Cat. # 74134 LunaScript RT SuperMix Kit New England Biolabs Cat. # M3010 (Continued on next page) Current Biology 35, 3570–3586.e1–e7, August 4, 2025 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Luna Universal qPCR Master Mix New England Biolabs Cat. # M3003 Experimental models: Cell lines melan-pa: Bloc1S6 pa/pa Functional Genomics Cell Bank at City St. George’s University of London N/A melan-mu: Bloc1S5 mu/mu Functional Genomics Cell Bank at City St. George’s University of London N/A melan-a: a/a Functional Genomics Cell Bank at City St. George’s University of London N/A melan-Ink4a-Arf1: Ink4a-Arf -/- Functional Genomics Cell Bank at City St. George’s University of London N/A melan-c2: Tyr c-2J /Tyr c-2J Functional Genomics Cell Bank at City St. George’s University of London N/A melan-mu:MuHA Functional Genomics Cell Bank at City St. George’s University of London N/A Oligonucleotides TYR Fwd: 5 ’ CGA TGG AAC ACC TGA GGG AC Integrated DNA Technologies N/A TYR Rev:5 ’ AGT GGA CTG GCA AAT CCT TCC Integrated DNA Technologies N/A Atp5b primer set (Anchor nucelotide: 115) Primer Design Ltd. geNorm kit (Discontinued) Ubc primer set (Anchor nucelotide: 2627) Primer Design Ltd. geNorm kit (Discontinued) B2m primer set (Anchor nucelotide: 202) Primer Design Ltd. geNorm kit (Discontinued) Sdha primer set (Anchor nucelotide: 2018) Primer Design Ltd. geNorm kit (Discontinued) Recombinant DNA pCR3/OCA2-HA Michael Marks N/A pCI-HA-SLC45A2 Michael Marks N/A pN1-LAMP1-tdTomato Tom Carter N/A Software and algorithms GraphPad Prism (version 10.2.1) GraphPad https://www.graphpad.com/ ImageJ NIH https://imagej.nih.gov/ij/ Photoshop CS6 (Version 13.0) Adobe Systems N/A NIS Elements (Version 4.2) Nikon N/A Image Studio (Version 5.2) Li-Cor N/A AMT Capture Engine software (Version V602.580.20d) Advanced Microscopy Techniques N/A Other RPMI-1640 Medium Sigma-Alrich Cat. # R7509 Fetal Bovine Serum Gibco Cat. # 10270106 Dextran, Oregon Green 488; 10,000 MW Invitrogen Cat. # D7170 L-Glutamine Gibco Cat. # 25030081 Penicillin-Streptomycin (10,000 U/mL) Gibco Cat. # 15140122 HEPES Gibco Cat. # 15630056 Protease Inhibitor cocktail Roche Cat. # 11697498001 Immobilon-FL PVDF Membrane Millipore Cat. # IPFL85R Nitrocellulose membrane, 0.45 μm BioRad Cat. # 1620115 CitiFluor AF1 mounting solution Agar Scientific Cat. # AGR1320 Copper mesh grids Agar Scientific Cat. # AGG2415C 35 mm Dish with No. 1.5 Gridded Coverslip, 14 mm Glass Diameter MatTek Cat. # P35G-1.5-14-C-GRD CitiFluor AF1 mounting solution Agar Scientific Cat. #AGR1320 Black 96 well plate Greiner Bio-One Cat. #655900 e2 Current Biology 35, 3570–3586.e1–e7, August 4, 2025 ..

    Recombinant:

    Article Title: Reprogramming of endolysosomes for melanogenesis in BLOC-1-deficient melanocytes.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies HA tag Abcam Cat. # ab9110; RRID:AB_307019 TYRP1 ATCC Cat. # TA99/Mel-5 LAMP2 Developmental Studies Hybridoma Bank Cat. # GL2A7; RRID:AB_2314734 Goat anti-mouse AlexaFluor488 Invitrogen Cat. # A-11001; RRID:AB_2534069 Goat anti-rabbit AlexaFluor568 Invitrogen Cat. # A-11011; RRID:AB_143157 Goat anti-rat AlexaFluor568 Invitrogen Cat. # A-11077; RRID:AB_141874 αPep7h (anti-TYR) Marks lab N/A αPep13h (anti-PMEL) Marks lab N/A TYRP1 Santa Cruz Biotechnology Cat. # sc-166857; RRID:AB_2211147 Actin Sigma-Aldrich Cat. # A5060; RRID:AB_476738 γ-tubulin Sigma Cat. # T6557; RRID:AB_477584 Goat anti-mouse IgG-IRDye 800CW Li-Cor Cat. # 926-32210; RRID:AB_621842 Goat anti-Rabbit IgG-IR Dye 680R Li-Cor Cat. # 926-68071; RRID:AB_10956166 Donkey anti-mouse IgG (H+L) AlexaFluor790 Jackson ImmunoResearch Cat. # 715-655-151; RRID:AB_2340871 Donkey anti-rabbit IgG (H+L) AlexaFluor680 Jackson ImmunoResearch Cat. # 711-625-152; RRID:AB_2340627 Chemicals, peptides, and recombinant proteins 12-0-tetradecanoyl phorbol acetate (TPA) Sigma-Aldrich Cat. # P8139 4-Norleucine,7-D-phenylalanine-alphamelanocyte-stimulating hormone (NDP-MSH) Sigma-Aldrich Cat. # M8764 Forskolin Sigma-Aldrich Cat. # F6886 Cholera toxin Sigma-Aldrich Cat. # C8052 Bafilomycin A1 Sigma-Aldrich Cat. # B1793 Cycloheximide Sigma-Aldrich Cat. # C1988-1G L-Methionine methyl ester hydrochloride Thermo Scientific Chemicals Cat. # A15952.14 Monensin Sigma-Aldrich Cat. # M5273 Nigericin Sigma-Aldrich Cat. # N7143 Sodium cacodylate Sigma-Alrich Cat. # C0250 Osmium Tetroxide Agar Scientific Cat. # AGR1019 Propylene oxide Sigma-Aldrich Cat. # 82320 Agar 100 Resin Kit Agar Scientific Cat. # AGR1031 Uranyl acetate Agar Scientific Cat. # AGR1260A Lead citrate Agar Scientific Cat. # AGR1210 Dodecenyl succinic anhydride Agar Scientific Cat. #R1053 Methyl nadic anhydride Agar Scientific Cat. #R1083 Benzyl dimethylamine Agar Scientific Cat. #AGR1062 Potassium ferricyanide Sigma-Aldrich Cat. # 702587 Tannic acid TAAB Laboratories Equipment Limited Cat. # T046 Sodium sulphate Sigma-Aldrich Cat. # 239313 TAAB 812 Resin kit TAAB Laboratories Equipment Limited Cat. # T024/1 Lipofectamine 3000 ThermoFisher Scientific Cat. #L3000015 Critical commercial assays RNeasy Plus Mini Kit Qiagen Cat. # 74134 LunaScript RT SuperMix Kit New England Biolabs Cat. # M3010 (Continued on next page) Current Biology 35, 3570–3586.e1–e7, August 4, 2025 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Luna Universal qPCR Master Mix New England Biolabs Cat. # M3003 Experimental models: Cell lines melan-pa: Bloc1S6 pa/pa Functional Genomics Cell Bank at City St. George’s University of London N/A melan-mu: Bloc1S5 mu/mu Functional Genomics Cell Bank at City St. George’s University of London N/A melan-a: a/a Functional Genomics Cell Bank at City St. George’s University of London N/A melan-Ink4a-Arf1: Ink4a-Arf -/- Functional Genomics Cell Bank at City St. George’s University of London N/A melan-c2: Tyr c-2J /Tyr c-2J Functional Genomics Cell Bank at City St. George’s University of London N/A melan-mu:MuHA Functional Genomics Cell Bank at City St. George’s University of London N/A Oligonucleotides TYR Fwd: 5 ’ CGA TGG AAC ACC TGA GGG AC Integrated DNA Technologies N/A TYR Rev:5 ’ AGT GGA CTG GCA AAT CCT TCC Integrated DNA Technologies N/A Atp5b primer set (Anchor nucelotide: 115) Primer Design Ltd. geNorm kit (Discontinued) Ubc primer set (Anchor nucelotide: 2627) Primer Design Ltd. geNorm kit (Discontinued) B2m primer set (Anchor nucelotide: 202) Primer Design Ltd. geNorm kit (Discontinued) Sdha primer set (Anchor nucelotide: 2018) Primer Design Ltd. geNorm kit (Discontinued) Recombinant DNA pCR3/OCA2-HA Michael Marks N/A pCI-HA-SLC45A2 Michael Marks N/A pN1-LAMP1-tdTomato Tom Carter N/A Software and algorithms GraphPad Prism (version 10.2.1) GraphPad https://www.graphpad.com/ ImageJ NIH https://imagej.nih.gov/ij/ Photoshop CS6 (Version 13.0) Adobe Systems N/A NIS Elements (Version 4.2) Nikon N/A Image Studio (Version 5.2) Li-Cor N/A AMT Capture Engine software (Version V602.580.20d) Advanced Microscopy Techniques N/A Other RPMI-1640 Medium Sigma-Alrich Cat. # R7509 Fetal Bovine Serum Gibco Cat. # 10270106 Dextran, Oregon Green 488; 10,000 MW Invitrogen Cat. # D7170 L-Glutamine Gibco Cat. # 25030081 Penicillin-Streptomycin (10,000 U/mL) Gibco Cat. # 15140122 HEPES Gibco Cat. # 15630056 Protease Inhibitor cocktail Roche Cat. # 11697498001 Immobilon-FL PVDF Membrane Millipore Cat. # IPFL85R Nitrocellulose membrane, 0.45 μm BioRad Cat. # 1620115 CitiFluor AF1 mounting solution Agar Scientific Cat. # AGR1320 Copper mesh grids Agar Scientific Cat. # AGG2415C 35 mm Dish with No. 1.5 Gridded Coverslip, 14 mm Glass Diameter MatTek Cat. # P35G-1.5-14-C-GRD CitiFluor AF1 mounting solution Agar Scientific Cat. #AGR1320 Black 96 well plate Greiner Bio-One Cat. #655900 e2 Current Biology 35, 3570–3586.e1–e7, August 4, 2025 ..

    Software:

    Article Title: Reprogramming of endolysosomes for melanogenesis in BLOC-1-deficient melanocytes.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies HA tag Abcam Cat. # ab9110; RRID:AB_307019 TYRP1 ATCC Cat. # TA99/Mel-5 LAMP2 Developmental Studies Hybridoma Bank Cat. # GL2A7; RRID:AB_2314734 Goat anti-mouse AlexaFluor488 Invitrogen Cat. # A-11001; RRID:AB_2534069 Goat anti-rabbit AlexaFluor568 Invitrogen Cat. # A-11011; RRID:AB_143157 Goat anti-rat AlexaFluor568 Invitrogen Cat. # A-11077; RRID:AB_141874 αPep7h (anti-TYR) Marks lab N/A αPep13h (anti-PMEL) Marks lab N/A TYRP1 Santa Cruz Biotechnology Cat. # sc-166857; RRID:AB_2211147 Actin Sigma-Aldrich Cat. # A5060; RRID:AB_476738 γ-tubulin Sigma Cat. # T6557; RRID:AB_477584 Goat anti-mouse IgG-IRDye 800CW Li-Cor Cat. # 926-32210; RRID:AB_621842 Goat anti-Rabbit IgG-IR Dye 680R Li-Cor Cat. # 926-68071; RRID:AB_10956166 Donkey anti-mouse IgG (H+L) AlexaFluor790 Jackson ImmunoResearch Cat. # 715-655-151; RRID:AB_2340871 Donkey anti-rabbit IgG (H+L) AlexaFluor680 Jackson ImmunoResearch Cat. # 711-625-152; RRID:AB_2340627 Chemicals, peptides, and recombinant proteins 12-0-tetradecanoyl phorbol acetate (TPA) Sigma-Aldrich Cat. # P8139 4-Norleucine,7-D-phenylalanine-alphamelanocyte-stimulating hormone (NDP-MSH) Sigma-Aldrich Cat. # M8764 Forskolin Sigma-Aldrich Cat. # F6886 Cholera toxin Sigma-Aldrich Cat. # C8052 Bafilomycin A1 Sigma-Aldrich Cat. # B1793 Cycloheximide Sigma-Aldrich Cat. # C1988-1G L-Methionine methyl ester hydrochloride Thermo Scientific Chemicals Cat. # A15952.14 Monensin Sigma-Aldrich Cat. # M5273 Nigericin Sigma-Aldrich Cat. # N7143 Sodium cacodylate Sigma-Alrich Cat. # C0250 Osmium Tetroxide Agar Scientific Cat. # AGR1019 Propylene oxide Sigma-Aldrich Cat. # 82320 Agar 100 Resin Kit Agar Scientific Cat. # AGR1031 Uranyl acetate Agar Scientific Cat. # AGR1260A Lead citrate Agar Scientific Cat. # AGR1210 Dodecenyl succinic anhydride Agar Scientific Cat. #R1053 Methyl nadic anhydride Agar Scientific Cat. #R1083 Benzyl dimethylamine Agar Scientific Cat. #AGR1062 Potassium ferricyanide Sigma-Aldrich Cat. # 702587 Tannic acid TAAB Laboratories Equipment Limited Cat. # T046 Sodium sulphate Sigma-Aldrich Cat. # 239313 TAAB 812 Resin kit TAAB Laboratories Equipment Limited Cat. # T024/1 Lipofectamine 3000 ThermoFisher Scientific Cat. #L3000015 Critical commercial assays RNeasy Plus Mini Kit Qiagen Cat. # 74134 LunaScript RT SuperMix Kit New England Biolabs Cat. # M3010 (Continued on next page) Current Biology 35, 3570–3586.e1–e7, August 4, 2025 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Luna Universal qPCR Master Mix New England Biolabs Cat. # M3003 Experimental models: Cell lines melan-pa: Bloc1S6 pa/pa Functional Genomics Cell Bank at City St. George’s University of London N/A melan-mu: Bloc1S5 mu/mu Functional Genomics Cell Bank at City St. George’s University of London N/A melan-a: a/a Functional Genomics Cell Bank at City St. George’s University of London N/A melan-Ink4a-Arf1: Ink4a-Arf -/- Functional Genomics Cell Bank at City St. George’s University of London N/A melan-c2: Tyr c-2J /Tyr c-2J Functional Genomics Cell Bank at City St. George’s University of London N/A melan-mu:MuHA Functional Genomics Cell Bank at City St. George’s University of London N/A Oligonucleotides TYR Fwd: 5 ’ CGA TGG AAC ACC TGA GGG AC Integrated DNA Technologies N/A TYR Rev:5 ’ AGT GGA CTG GCA AAT CCT TCC Integrated DNA Technologies N/A Atp5b primer set (Anchor nucelotide: 115) Primer Design Ltd. geNorm kit (Discontinued) Ubc primer set (Anchor nucelotide: 2627) Primer Design Ltd. geNorm kit (Discontinued) B2m primer set (Anchor nucelotide: 202) Primer Design Ltd. geNorm kit (Discontinued) Sdha primer set (Anchor nucelotide: 2018) Primer Design Ltd. geNorm kit (Discontinued) Recombinant DNA pCR3/OCA2-HA Michael Marks N/A pCI-HA-SLC45A2 Michael Marks N/A pN1-LAMP1-tdTomato Tom Carter N/A Software and algorithms GraphPad Prism (version 10.2.1) GraphPad https://www.graphpad.com/ ImageJ NIH https://imagej.nih.gov/ij/ Photoshop CS6 (Version 13.0) Adobe Systems N/A NIS Elements (Version 4.2) Nikon N/A Image Studio (Version 5.2) Li-Cor N/A AMT Capture Engine software (Version V602.580.20d) Advanced Microscopy Techniques N/A Other RPMI-1640 Medium Sigma-Alrich Cat. # R7509 Fetal Bovine Serum Gibco Cat. # 10270106 Dextran, Oregon Green 488; 10,000 MW Invitrogen Cat. # D7170 L-Glutamine Gibco Cat. # 25030081 Penicillin-Streptomycin (10,000 U/mL) Gibco Cat. # 15140122 HEPES Gibco Cat. # 15630056 Protease Inhibitor cocktail Roche Cat. # 11697498001 Immobilon-FL PVDF Membrane Millipore Cat. # IPFL85R Nitrocellulose membrane, 0.45 μm BioRad Cat. # 1620115 CitiFluor AF1 mounting solution Agar Scientific Cat. # AGR1320 Copper mesh grids Agar Scientific Cat. # AGG2415C 35 mm Dish with No. 1.5 Gridded Coverslip, 14 mm Glass Diameter MatTek Cat. # P35G-1.5-14-C-GRD CitiFluor AF1 mounting solution Agar Scientific Cat. #AGR1320 Black 96 well plate Greiner Bio-One Cat. #655900 e2 Current Biology 35, 3570–3586.e1–e7, August 4, 2025 ..

    Protease Inhibitor:

    Article Title: Reprogramming of endolysosomes for melanogenesis in BLOC-1-deficient melanocytes.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies HA tag Abcam Cat. # ab9110; RRID:AB_307019 TYRP1 ATCC Cat. # TA99/Mel-5 LAMP2 Developmental Studies Hybridoma Bank Cat. # GL2A7; RRID:AB_2314734 Goat anti-mouse AlexaFluor488 Invitrogen Cat. # A-11001; RRID:AB_2534069 Goat anti-rabbit AlexaFluor568 Invitrogen Cat. # A-11011; RRID:AB_143157 Goat anti-rat AlexaFluor568 Invitrogen Cat. # A-11077; RRID:AB_141874 αPep7h (anti-TYR) Marks lab N/A αPep13h (anti-PMEL) Marks lab N/A TYRP1 Santa Cruz Biotechnology Cat. # sc-166857; RRID:AB_2211147 Actin Sigma-Aldrich Cat. # A5060; RRID:AB_476738 γ-tubulin Sigma Cat. # T6557; RRID:AB_477584 Goat anti-mouse IgG-IRDye 800CW Li-Cor Cat. # 926-32210; RRID:AB_621842 Goat anti-Rabbit IgG-IR Dye 680R Li-Cor Cat. # 926-68071; RRID:AB_10956166 Donkey anti-mouse IgG (H+L) AlexaFluor790 Jackson ImmunoResearch Cat. # 715-655-151; RRID:AB_2340871 Donkey anti-rabbit IgG (H+L) AlexaFluor680 Jackson ImmunoResearch Cat. # 711-625-152; RRID:AB_2340627 Chemicals, peptides, and recombinant proteins 12-0-tetradecanoyl phorbol acetate (TPA) Sigma-Aldrich Cat. # P8139 4-Norleucine,7-D-phenylalanine-alphamelanocyte-stimulating hormone (NDP-MSH) Sigma-Aldrich Cat. # M8764 Forskolin Sigma-Aldrich Cat. # F6886 Cholera toxin Sigma-Aldrich Cat. # C8052 Bafilomycin A1 Sigma-Aldrich Cat. # B1793 Cycloheximide Sigma-Aldrich Cat. # C1988-1G L-Methionine methyl ester hydrochloride Thermo Scientific Chemicals Cat. # A15952.14 Monensin Sigma-Aldrich Cat. # M5273 Nigericin Sigma-Aldrich Cat. # N7143 Sodium cacodylate Sigma-Alrich Cat. # C0250 Osmium Tetroxide Agar Scientific Cat. # AGR1019 Propylene oxide Sigma-Aldrich Cat. # 82320 Agar 100 Resin Kit Agar Scientific Cat. # AGR1031 Uranyl acetate Agar Scientific Cat. # AGR1260A Lead citrate Agar Scientific Cat. # AGR1210 Dodecenyl succinic anhydride Agar Scientific Cat. #R1053 Methyl nadic anhydride Agar Scientific Cat. #R1083 Benzyl dimethylamine Agar Scientific Cat. #AGR1062 Potassium ferricyanide Sigma-Aldrich Cat. # 702587 Tannic acid TAAB Laboratories Equipment Limited Cat. # T046 Sodium sulphate Sigma-Aldrich Cat. # 239313 TAAB 812 Resin kit TAAB Laboratories Equipment Limited Cat. # T024/1 Lipofectamine 3000 ThermoFisher Scientific Cat. #L3000015 Critical commercial assays RNeasy Plus Mini Kit Qiagen Cat. # 74134 LunaScript RT SuperMix Kit New England Biolabs Cat. # M3010 (Continued on next page) Current Biology 35, 3570–3586.e1–e7, August 4, 2025 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Luna Universal qPCR Master Mix New England Biolabs Cat. # M3003 Experimental models: Cell lines melan-pa: Bloc1S6 pa/pa Functional Genomics Cell Bank at City St. George’s University of London N/A melan-mu: Bloc1S5 mu/mu Functional Genomics Cell Bank at City St. George’s University of London N/A melan-a: a/a Functional Genomics Cell Bank at City St. George’s University of London N/A melan-Ink4a-Arf1: Ink4a-Arf -/- Functional Genomics Cell Bank at City St. George’s University of London N/A melan-c2: Tyr c-2J /Tyr c-2J Functional Genomics Cell Bank at City St. George’s University of London N/A melan-mu:MuHA Functional Genomics Cell Bank at City St. George’s University of London N/A Oligonucleotides TYR Fwd: 5 ’ CGA TGG AAC ACC TGA GGG AC Integrated DNA Technologies N/A TYR Rev:5 ’ AGT GGA CTG GCA AAT CCT TCC Integrated DNA Technologies N/A Atp5b primer set (Anchor nucelotide: 115) Primer Design Ltd. geNorm kit (Discontinued) Ubc primer set (Anchor nucelotide: 2627) Primer Design Ltd. geNorm kit (Discontinued) B2m primer set (Anchor nucelotide: 202) Primer Design Ltd. geNorm kit (Discontinued) Sdha primer set (Anchor nucelotide: 2018) Primer Design Ltd. geNorm kit (Discontinued) Recombinant DNA pCR3/OCA2-HA Michael Marks N/A pCI-HA-SLC45A2 Michael Marks N/A pN1-LAMP1-tdTomato Tom Carter N/A Software and algorithms GraphPad Prism (version 10.2.1) GraphPad https://www.graphpad.com/ ImageJ NIH https://imagej.nih.gov/ij/ Photoshop CS6 (Version 13.0) Adobe Systems N/A NIS Elements (Version 4.2) Nikon N/A Image Studio (Version 5.2) Li-Cor N/A AMT Capture Engine software (Version V602.580.20d) Advanced Microscopy Techniques N/A Other RPMI-1640 Medium Sigma-Alrich Cat. # R7509 Fetal Bovine Serum Gibco Cat. # 10270106 Dextran, Oregon Green 488; 10,000 MW Invitrogen Cat. # D7170 L-Glutamine Gibco Cat. # 25030081 Penicillin-Streptomycin (10,000 U/mL) Gibco Cat. # 15140122 HEPES Gibco Cat. # 15630056 Protease Inhibitor cocktail Roche Cat. # 11697498001 Immobilon-FL PVDF Membrane Millipore Cat. # IPFL85R Nitrocellulose membrane, 0.45 μm BioRad Cat. # 1620115 CitiFluor AF1 mounting solution Agar Scientific Cat. # AGR1320 Copper mesh grids Agar Scientific Cat. # AGG2415C 35 mm Dish with No. 1.5 Gridded Coverslip, 14 mm Glass Diameter MatTek Cat. # P35G-1.5-14-C-GRD CitiFluor AF1 mounting solution Agar Scientific Cat. #AGR1320 Black 96 well plate Greiner Bio-One Cat. #655900 e2 Current Biology 35, 3570–3586.e1–e7, August 4, 2025 ..

    Membrane:

    Article Title: Reprogramming of endolysosomes for melanogenesis in BLOC-1-deficient melanocytes.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies HA tag Abcam Cat. # ab9110; RRID:AB_307019 TYRP1 ATCC Cat. # TA99/Mel-5 LAMP2 Developmental Studies Hybridoma Bank Cat. # GL2A7; RRID:AB_2314734 Goat anti-mouse AlexaFluor488 Invitrogen Cat. # A-11001; RRID:AB_2534069 Goat anti-rabbit AlexaFluor568 Invitrogen Cat. # A-11011; RRID:AB_143157 Goat anti-rat AlexaFluor568 Invitrogen Cat. # A-11077; RRID:AB_141874 αPep7h (anti-TYR) Marks lab N/A αPep13h (anti-PMEL) Marks lab N/A TYRP1 Santa Cruz Biotechnology Cat. # sc-166857; RRID:AB_2211147 Actin Sigma-Aldrich Cat. # A5060; RRID:AB_476738 γ-tubulin Sigma Cat. # T6557; RRID:AB_477584 Goat anti-mouse IgG-IRDye 800CW Li-Cor Cat. # 926-32210; RRID:AB_621842 Goat anti-Rabbit IgG-IR Dye 680R Li-Cor Cat. # 926-68071; RRID:AB_10956166 Donkey anti-mouse IgG (H+L) AlexaFluor790 Jackson ImmunoResearch Cat. # 715-655-151; RRID:AB_2340871 Donkey anti-rabbit IgG (H+L) AlexaFluor680 Jackson ImmunoResearch Cat. # 711-625-152; RRID:AB_2340627 Chemicals, peptides, and recombinant proteins 12-0-tetradecanoyl phorbol acetate (TPA) Sigma-Aldrich Cat. # P8139 4-Norleucine,7-D-phenylalanine-alphamelanocyte-stimulating hormone (NDP-MSH) Sigma-Aldrich Cat. # M8764 Forskolin Sigma-Aldrich Cat. # F6886 Cholera toxin Sigma-Aldrich Cat. # C8052 Bafilomycin A1 Sigma-Aldrich Cat. # B1793 Cycloheximide Sigma-Aldrich Cat. # C1988-1G L-Methionine methyl ester hydrochloride Thermo Scientific Chemicals Cat. # A15952.14 Monensin Sigma-Aldrich Cat. # M5273 Nigericin Sigma-Aldrich Cat. # N7143 Sodium cacodylate Sigma-Alrich Cat. # C0250 Osmium Tetroxide Agar Scientific Cat. # AGR1019 Propylene oxide Sigma-Aldrich Cat. # 82320 Agar 100 Resin Kit Agar Scientific Cat. # AGR1031 Uranyl acetate Agar Scientific Cat. # AGR1260A Lead citrate Agar Scientific Cat. # AGR1210 Dodecenyl succinic anhydride Agar Scientific Cat. #R1053 Methyl nadic anhydride Agar Scientific Cat. #R1083 Benzyl dimethylamine Agar Scientific Cat. #AGR1062 Potassium ferricyanide Sigma-Aldrich Cat. # 702587 Tannic acid TAAB Laboratories Equipment Limited Cat. # T046 Sodium sulphate Sigma-Aldrich Cat. # 239313 TAAB 812 Resin kit TAAB Laboratories Equipment Limited Cat. # T024/1 Lipofectamine 3000 ThermoFisher Scientific Cat. #L3000015 Critical commercial assays RNeasy Plus Mini Kit Qiagen Cat. # 74134 LunaScript RT SuperMix Kit New England Biolabs Cat. # M3010 (Continued on next page) Current Biology 35, 3570–3586.e1–e7, August 4, 2025 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Luna Universal qPCR Master Mix New England Biolabs Cat. # M3003 Experimental models: Cell lines melan-pa: Bloc1S6 pa/pa Functional Genomics Cell Bank at City St. George’s University of London N/A melan-mu: Bloc1S5 mu/mu Functional Genomics Cell Bank at City St. George’s University of London N/A melan-a: a/a Functional Genomics Cell Bank at City St. George’s University of London N/A melan-Ink4a-Arf1: Ink4a-Arf -/- Functional Genomics Cell Bank at City St. George’s University of London N/A melan-c2: Tyr c-2J /Tyr c-2J Functional Genomics Cell Bank at City St. George’s University of London N/A melan-mu:MuHA Functional Genomics Cell Bank at City St. George’s University of London N/A Oligonucleotides TYR Fwd: 5 ’ CGA TGG AAC ACC TGA GGG AC Integrated DNA Technologies N/A TYR Rev:5 ’ AGT GGA CTG GCA AAT CCT TCC Integrated DNA Technologies N/A Atp5b primer set (Anchor nucelotide: 115) Primer Design Ltd. geNorm kit (Discontinued) Ubc primer set (Anchor nucelotide: 2627) Primer Design Ltd. geNorm kit (Discontinued) B2m primer set (Anchor nucelotide: 202) Primer Design Ltd. geNorm kit (Discontinued) Sdha primer set (Anchor nucelotide: 2018) Primer Design Ltd. geNorm kit (Discontinued) Recombinant DNA pCR3/OCA2-HA Michael Marks N/A pCI-HA-SLC45A2 Michael Marks N/A pN1-LAMP1-tdTomato Tom Carter N/A Software and algorithms GraphPad Prism (version 10.2.1) GraphPad https://www.graphpad.com/ ImageJ NIH https://imagej.nih.gov/ij/ Photoshop CS6 (Version 13.0) Adobe Systems N/A NIS Elements (Version 4.2) Nikon N/A Image Studio (Version 5.2) Li-Cor N/A AMT Capture Engine software (Version V602.580.20d) Advanced Microscopy Techniques N/A Other RPMI-1640 Medium Sigma-Alrich Cat. # R7509 Fetal Bovine Serum Gibco Cat. # 10270106 Dextran, Oregon Green 488; 10,000 MW Invitrogen Cat. # D7170 L-Glutamine Gibco Cat. # 25030081 Penicillin-Streptomycin (10,000 U/mL) Gibco Cat. # 15140122 HEPES Gibco Cat. # 15630056 Protease Inhibitor cocktail Roche Cat. # 11697498001 Immobilon-FL PVDF Membrane Millipore Cat. # IPFL85R Nitrocellulose membrane, 0.45 μm BioRad Cat. # 1620115 CitiFluor AF1 mounting solution Agar Scientific Cat. # AGR1320 Copper mesh grids Agar Scientific Cat. # AGG2415C 35 mm Dish with No. 1.5 Gridded Coverslip, 14 mm Glass Diameter MatTek Cat. # P35G-1.5-14-C-GRD CitiFluor AF1 mounting solution Agar Scientific Cat. #AGR1320 Black 96 well plate Greiner Bio-One Cat. #655900 e2 Current Biology 35, 3570–3586.e1–e7, August 4, 2025 ..



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